Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myotilin CRISPR/Cas9 KO Plasmid (h): sc-406960
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myotilin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myotilin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myotilin Antibody (E-10): sc-393957
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myotilin CRISPR/Cas9 KO Plasmid (h) | sc-406960 | 20 µg | $397.00 |
Overview
MYOT encodes myotilin, a Z-disc–localized cytoskeletal protein that binds actin-associated components to support sarcomere organization and mechanical stability in striated muscle. Myotilin participates in myofibrillogenesis and maintenance of Z-disc architecture through interactions with proteins such as α-actinin and filamin C, influencing cytoskeletal signaling and mechanotransduction. Disruption of MYOT function is linked to myofibrillar myopathies and related muscle disorders characterized by Z-disc disintegration and protein aggregation, making it relevant for studies of muscle integrity and stress responses. As a structural hub in contractile tissue, myotilin is frequently examined in pathways governing muscle differentiation, protein quality control, and cytoskeletal remodeling.
Myotilin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYOT gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYOT together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYOT open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myotilin protein expression.
This CRISPR knockout system enables efficient generation of MYOT-deficient cell models for investigation of Myotilin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYOT exon(s) critical for Myotilin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYOT genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myotilin CRISPR/Cas9 KO Plasmid (h) and Myotilin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYOT locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myotilin HDR Plasmid (h) and Myotilin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYOT homology arms to support homology-directed repair at defined MYOT target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.