MTF-2 CRISPR/Cas9 KO Plasmid (h): sc-411609
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MTF-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MTF-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MTF-2 CRISPR/Cas9 KO Plasmid (h) | sc-411609 | 20 µg | $397.00 |
Overview
MTF2 (metal response element binding transcription factor 2) encodes MTF-2, a Polycomb group–associated chromatin regulator that participates in transcriptional repression programs controlling lineage specification and cell-state stability. MTF-2 is linked to PRC2 function and H3K27 methylation–dependent silencing at developmental loci, influencing chromatin accessibility and long-range gene regulatory networks. Through these epigenetic mechanisms, MTF2 contributes to control of proliferation, differentiation, and stress-responsive transcriptional outputs. Dysregulated MTF2 activity has been reported in studies of cancer biology and developmental disorders, where altered Polycomb targeting can perturb gene expression landscapes.
MTF-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MTF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MTF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MTF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MTF-2 protein expression.
This CRISPR knockout system enables efficient generation of MTF2-deficient cell models for investigation of MTF-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MTF2 exon(s) critical for MTF-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MTF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MTF-2 CRISPR/Cas9 KO Plasmid (h) and MTF-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MTF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MTF-2 HDR Plasmid (h) and MTF-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MTF2 homology arms to support homology-directed repair at defined MTF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.