Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MSG1 Double Nickase Plasmid (m): sc-419669-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MSG1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MSG1 Double Nickase Plasmid (m) and MSG1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cited1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MSG1 Antibody (D-7): sc-393585
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSG1 Double Nickase Plasmid (m) | sc-419669-NIC | 20 µg | $410.00 | |||
MSG1 Double Nickase Plasmid (m2) | sc-419669-NIC-2 | 20 µg | $410.00 |
Mouse Cited1 encodes MSG1, a transcriptional co-regulator that modulates gene expression programs governing cell fate decisions and differentiation. MSG1 interacts with nuclear transcriptional machinery, including CBP/p300-associated pathways, to influence chromatin-dependent regulation of developmental and stress-responsive genes. In embryonic and stem/progenitor contexts, Cited1 contributes to lineage specification and tissue patterning, while in somatic settings it can shape signaling outputs from pathways that converge on transcriptional control. Dysregulated CITED family activity has been linked in the literature to altered differentiation states and oncogenic transcriptional networks, making Cited1 a useful target for mechanistic studies of gene regulation.
MSG1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cited1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cited1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cited1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cited1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.