MSG1 Double Nickase Plasmid (h): sc-404984-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MSG1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MSG1 Double Nickase Plasmid (h) and MSG1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CITED1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MSG1 Antibody (35-W): sc-101002
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSG1 Double Nickase Plasmid (h) | sc-404984-NIC | 20 µg | $410.00 | |||
MSG1 Double Nickase Plasmid (h2) | sc-404984-NIC-2 | 20 µg | $410.00 |
CITED1 (also known as MSG1) encodes a nuclear transcriptional co-regulator that binds the CBP/p300 acetyltransferase complex to modulate gene expression programs downstream of developmental and hormonal cues. It participates in transcriptional control linked to TGF-β/BMP signaling dynamics, hypoxia-associated networks via HIF pathway crosstalk, and regulation of cell fate decisions including differentiation and proliferation. In human cells, altered CITED1 expression has been associated with dysregulated transcriptional states observed in several disease contexts, particularly in cancers and conditions involving aberrant growth factor signaling. These attributes make CITED1/MSG1 a useful target for dissecting enhancer–promoter regulation and signaling-dependent transcriptional rewiring.
MSG1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CITED1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CITED1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CITED1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CITED1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.