Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MMS19 CRISPR/Cas9 KO Plasmid (h): sc-405964
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMS19 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MMS19 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MMS19 Antibody (G-12): sc-390028
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMS19 CRISPR/Cas9 KO Plasmid (h) | sc-405964 | 20 µg | $397.00 |
Overview
MMS19 encodes MMS19, a conserved factor implicated in genome maintenance through its roles in cytosolic iron–sulfur (Fe–S) cluster assembly and the maturation of Fe–S–dependent nuclear proteins involved in DNA replication and repair. By supporting the functional integrity of multiple DNA metabolism enzymes, MMS19 contributes to replication stress tolerance, DNA damage response signaling, and overall chromosomal stability. Perturbation of MMS19 is therefore relevant to pathways that safeguard genome integrity and can influence cellular sensitivity to genotoxic stressors. Dysregulated MMS19-linked processes are of interest in studies of mutation accumulation, checkpoint control, and mechanisms underlying genome instability phenotypes observed in disease-associated contexts.
MMS19 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MMS19 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MMS19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MMS19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MMS19 protein expression.
This CRISPR knockout system enables efficient generation of MMS19-deficient cell models for investigation of MMS19 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MMS19 exon(s) critical for MMS19 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MMS19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MMS19 CRISPR/Cas9 KO Plasmid (h) and MMS19 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MMS19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MMS19 HDR Plasmid (h) and MMS19 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MMS19 homology arms to support homology-directed repair at defined MMS19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.