MKP-1/DUSP1 Double Nickase Plasmid (m): sc-422507-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MKP-1/DUSP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MKP-1/DUSP1 Double Nickase Plasmid (m) and MKP-1/DUSP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Dusp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MKP-1/DUSP1 Antibody (E-6): sc-373841
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-1/DUSP1 Double Nickase Plasmid (m) | sc-422507-NIC | 20 µg | $410.00 | |||
MKP-1/DUSP1 Double Nickase Plasmid (m2) | sc-422507-NIC-2 | 20 µg | $410.00 |
Dusp1 encodes mitogen-activated protein kinase phosphatase-1 (MKP-1/DUSP1), a dual-specificity phosphatase that dephosphorylates and inactivates MAPKs including ERK, JNK, and p38. As an inducible immediate-early regulator, MKP-1 provides negative feedback control of stress- and cytokine-driven signaling, shaping transcriptional programs linked to inflammation, apoptosis, and cell-cycle progression. In mouse cells, Dusp1 is commonly studied in pathways downstream of TLR ligands, growth factors, and oxidative stress where it limits MAPK amplitude and duration. Dysregulated DUSP1 activity has been associated with inflammatory phenotypes and altered stress responses relevant to models of immune dysregulation, metabolic disease, and cancer biology.
MKP-1/DUSP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Dusp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Dusp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Dusp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Dusp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.