Mitoferrin 2 Double Nickase Plasmid (m): sc-434219-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mitoferrin 2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mitoferrin 2 Double Nickase Plasmid (m) and Mitoferrin 2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc25a28. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mitoferrin 2 Double Nickase Plasmid (m) | sc-434219-NIC | 20 µg | $410.00 | |||
Mitoferrin 2 Double Nickase Plasmid (m2) | sc-434219-NIC-2 | 20 µg | $410.00 |
Slc25a28 encodes Mitoferrin 2, an inner mitochondrial membrane carrier that imports ferrous iron into the mitochondrial matrix to support heme biosynthesis and iron–sulfur (Fe–S) cluster assembly. By controlling mitochondrial iron availability, Mitoferrin 2 helps coordinate oxidative phosphorylation, redox balance, and mitochondrial quality control pathways that are sensitive to iron-driven reactive oxygen species. Perturbation of this transporter can disrupt erythroid and non-erythroid iron utilization programs, leading to defects in mitochondrial metabolism and stress responses. As a conserved component of cellular iron homeostasis, Slc25a28 is frequently studied in contexts linking mitochondrial dysfunction with anemia-related mechanisms and neurodegeneration-relevant oxidative stress phenotypes in mouse models.
Mitoferrin 2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc25a28 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc25a28. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc25a28 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc25a28-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.