MGP Antibody (H-4): sc-271907

MGP Antibody (H-4) is a mouse monoclonal IgG2a antibody that detects Matrix Gla protein (MGP) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MGP plays a crucial role in regulating calcification within the extracellular matrix, particularly in cartilage and arterial tissues, which is vital for maintaining proper vascular health and preventing pathological calcification. This vitamin K-dependent protein is characterized by the presence of five to six residues of γ-carboxy-glutamic acid (Gla), an amino acid that binds calcium ions and is essential for MGP′s function. In humans, MGP consists of 84 amino acids along with a 19-amino-acid transmembrane signal peptide, while a truncated 77-residue form is found in bone extracts, likely resulting from enzymatic processing. MGP is predominantly expressed in the heart, kidney, and lung, with notable overexpression in the breast cancer cell line 600 PEI. Additionally, retinoic acid has been shown to induce MGP expression in chondrocytes, fibroblasts, and osteoblasts, highlighting MGP′s regulatory role in cellular processes. Importantly, mutations in the MGP gene can lead to Keutel syndrome, a condition associated with abnormal cartilage calcification, further underscoring MGP′s significance in extracellular matrix regulation. Anti-MGP antibody (H-4) is an essential tool for researchers investigating the intricate mechanisms of calcification and its implications in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MGP Antibody (H-4) References:

1. Phosphate-dependent regulation of MGP in osteoblasts: role of ERK1/2 and Fra-1.  |  Julien, M., et al. 2009. J Bone Miner Res. 24: 1856-68. PMID: 19419315
2. Matrix gla protein (MGP): an overexpressed and migration-promoting mesenchymal component in glioblastoma.  |  Mertsch, S., et al. 2009. BMC Cancer. 9: 302. PMID: 19712474
3. Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin.  |  Beazley, KE., et al. 2013. J Biol Chem. 288: 31400-8. PMID: 24036114
4. The Role of Matrix Gla Protein (MGP) in Vascular Calcification.  |  Bjørklund, G., et al. 2020. Curr Med Chem. 27: 1647-1660. PMID: 30009696
5. Association of Genetic Polymorphisms in the Matrix Gla Protein (MGP) Gene with Coronary Artery Disease and Serum MGP Levels.  |  Karsli-Ceppioglu, S., et al. 2019. Balkan J Med Genet. 22: 43-50. PMID: 31942416
6. Suppression MGP inhibits tumor proliferation and reverses oxaliplatin resistance in colorectal cancer.  |  Huang, C., et al. 2021. Biochem Pharmacol. 189: 114390. PMID: 33359068
7. MGP Regulates Perivascular Adipose-Derived Stem Cells Differentiation Toward Smooth Muscle Cells Via BMP2/SMAD Pathway Enhancing Neointimal Formation.  |  Ni, H., et al. 2022. Cell Transplant. 31: 9636897221075747. PMID: 35168405
8. MGP promotes CD8+ T cell exhaustion by activating the NF-κB pathway leading to liver metastasis of colorectal cancer.  |  Rong, D., et al. 2022. Int J Biol Sci. 18: 2345-2361. PMID: 35414780
9. Temperature-dependent Raman spectra and thermal expansion of MgP2O6.  |  Xue, W., et al. 2023. Spectrochim Acta A Mol Biomol Spectrosc. 303: 123239. PMID: 37562214
10. LGR4 attenuates MGP expression and suppresses EGFR activation-induced triple-negative breast cancer metastasis.  |  Li, Q., et al. 2024. Am J Cancer Res. 14: 3419-3432. PMID: 39113859

The preferred MGP antibody is MGP Antibody (A-11): sc-271906.