Met Antibody (B-2): sc-8057
- Met Antibody (B-2) is a mouse monoclonal IgG1 κ Met antibody, cited in 125 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1359-1379 at the C-terminus of c-Met p140 of mouse origin
- Met Antibody (B-2) is recommended for detection of Met of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Met Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Met Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Met Antibody (B-2) (see ordering information below).
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Met Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Met of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Met monoclonal antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Met, also known as c-Met, is a transmembrane receptor with intrinsic tyrosine kinase activity, playing a crucial role in cellular processes such as proliferation, motility, and morphogenesis. Met function is particularly important in the context of tissue regeneration and repair, as Met serves as the receptor for hepatocyte growth factor, a key player in liver regeneration. Dysregulation of c-Met signaling has been implicated in various cancers, making Met a significant target for therapeutic intervention. The c-Met oncogene was first identified through transfection analysis in NIH/3T3 cells derived from a chemically induced human osteogenic sarcoma, highlighting Met relevance in oncogenesis. Furthermore, the presence of tyrosine phosphorylation of Met in certain human gastric carcinoma cell lines underscores Met potential role in tumor progression and metastasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Met Antibody (B-2) References:
- c-Met and hepatocyte growth factor: potential as novel targets in cancer therapy. | Sattler, M. and Salgia, R. 2007. Curr Oncol Rep. 9: 102-8. PMID: 17288874
- In vivo detection of c-MET expression in a rat hepatocarcinogenesis model using molecularly targeted magnetic resonance imaging. | Towner, RA., et al. 2007. Mol Imaging. 6: 18-29. PMID: 17311762
- Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. | Bottaro, DP., et al. 1991. Science. 251: 802-4. PMID: 1846706
- Mechanism of met oncogene activation. | Park, M., et al. 1986. Cell. 45: 895-904. PMID: 2423252
- Characterization of the mouse met proto-oncogene. | Chan, AM., et al. 1988. Oncogene. 2: 593-9. PMID: 2838789
- Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts. | Cooper, CS., et al. 1986. EMBO J. 5: 2623-8. PMID: 3023053
- p145, a protein with associated tyrosine kinase activity in a human gastric carcinoma cell line. | Giordano, S., et al. 1988. Mol Cell Biol. 8: 3510-7. PMID: 3211149
- Targeting MET Dysregulation in Cancer. | Recondo, G., et al. 2020. Cancer Discov. 10: 922-934. PMID: 32532746
- The role of MET in chemotherapy resistance. | Wood, GE., et al. 2021. Oncogene. 40: 1927-1941. PMID: 33526881
- MET-Activating Ubiquitin Multimers. | Kawakami, N., et al. 2023. Angew Chem Int Ed Engl. 62: e202307157. PMID: 37450419
- Molecular cloning of a new transforming gene from a chemically transformed human cell line. | Cooper, CS., et al. Nature. 311: 29-33. PMID: 6590967
- Phosphorylation of serine 985 negatively regulates the hepatocyte growth factor receptor kinase. | Gandino, L., et al. 1994. J Biol Chem. 269: 1815-20. PMID: 8294430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Met Antibody (B-2) | sc-8057 | 200 µg/ml | $322.00 | |||
Met Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-526513 | 200 µg Ab; 10 µg BP | $361.00 | |||
Met Antibody (B-2): m-IgG1 BP-HRP Bundle | sc-531886 | 200 µg Ab; 20 µg BP | $361.00 | |||
Met Antibody (B-2) AC | sc-8057 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Met Antibody (B-2) HRP | sc-8057 HRP | 200 µg/ml | $322.00 | |||
Met Antibody (B-2) FITC | sc-8057 FITC | 200 µg/ml | $336.00 | |||
Met Antibody (B-2) PE | sc-8057 PE | 200 µg/ml | $349.00 | |||
Met Antibody (B-2) Alexa Fluor® 488 | sc-8057 AF488 | 200 µg/ml | $364.00 | |||
Met Antibody (B-2) Alexa Fluor® 546 | sc-8057 AF546 | 200 µg/ml | $364.00 | |||
Met Antibody (B-2) Alexa Fluor® 594 | sc-8057 AF594 | 200 µg/ml | $364.00 | |||
Met Antibody (B-2) Alexa Fluor® 647 | sc-8057 AF647 | 200 µg/ml | $364.00 | |||
Met Antibody (B-2) Alexa Fluor® 680 | sc-8057 AF680 | 200 µg/ml | $364.00 | |||
Met Antibody (B-2) Alexa Fluor® 790 | sc-8057 AF790 | 200 µg/ml | $364.00 | |||
Met (B-2) Neutralizing Peptide | sc-8057 P | 100 µg/0.5 ml | $69.00 |