Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MDH2 CRISPR/Cas9 KO Plasmid (h): sc-402825
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDH2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MDH2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MDH2 Antibody (1G12): sc-293474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDH2 CRISPR/Cas9 KO Plasmid (h) | sc-402825 | 20 µg | $397.00 |
Overview
MDH2 encodes mitochondrial malate dehydrogenase 2, a key NAD⁺-dependent enzyme that catalyzes the reversible conversion of malate to oxaloacetate in the tricarboxylic acid (TCA) cycle. By controlling mitochondrial NADH production and oxaloacetate availability, MDH2 helps coordinate oxidative metabolism, anaplerosis, and redox balance, and supports biosynthetic fluxes coupled to mitochondrial respiration. MDH2 function is tightly linked to metabolic reprogramming, reactive oxygen species handling, and mitochondrial quality control pathways that shape cellular fitness under nutrient or hypoxic stress. Altered MDH2 activity or expression has been implicated in disease-relevant phenotypes associated with mitochondrial dysfunction, including neurometabolic imbalance and tumor-associated metabolic dependencies.
MDH2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MDH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MDH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MDH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MDH2 protein expression.
This CRISPR knockout system enables efficient generation of MDH2-deficient cell models for investigation of MDH2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MDH2 exon(s) critical for MDH2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MDH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MDH2 CRISPR/Cas9 KO Plasmid (h) and MDH2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MDH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MDH2 HDR Plasmid (h) and MDH2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MDH2 homology arms to support homology-directed repair at defined MDH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.