Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCM5 CRISPR/Cas9 KO Plasmid (h): sc-401980
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MCM5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCM5 Antibody (E-10): sc-165994
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM5 CRISPR/Cas9 KO Plasmid (h) | sc-401980 | 20 µg | $397.00 |
Overview
MCM5 encodes a core subunit of the MCM2–7 helicase that licenses replication origins and drives DNA unwinding during S phase as part of the pre-replication and CMG (CDC45–MCM–GINS) complexes. By coordinating origin firing and replication fork progression, MCM5 contributes to genome stability and interfaces with DNA damage response and checkpoint pathways that restrain aberrant DNA synthesis. Dysregulated expression or activity of MCM family proteins is frequently linked to heightened proliferative capacity and replication stress signatures observed across multiple tumor contexts, supporting its use as a mechanistic marker in cell-cycle and genome maintenance studies.
MCM5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MCM5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MCM5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MCM5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MCM5 protein expression.
This CRISPR knockout system enables efficient generation of MCM5-deficient cell models for investigation of MCM5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MCM5 exon(s) critical for MCM5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MCM5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MCM5 CRISPR/Cas9 KO Plasmid (h) and MCM5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MCM5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MCM5 HDR Plasmid (h) and MCM5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MCM5 homology arms to support homology-directed repair at defined MCM5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.