Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MATH-3 CRISPR/Cas9 KO Plasmid (m): sc-419232
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MATH-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MATH-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MATH-3 Antibody (D-10): sc-393724
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MATH-3 CRISPR/Cas9 KO Plasmid (m) | sc-419232 | 20 µg | $397.00 |
Overview
Neurod4 encodes the basic helix-loop-helix transcription factor MATH-3, a neurogenic regulator that promotes neuronal lineage commitment and differentiation during mouse development. MATH-3 functions within transcriptional programs that integrate proneural bHLH networks and Notch-dependent decisions to coordinate cell-cycle exit, neuronal subtype specification, and maturation. By controlling downstream gene expression in developing neural tissues, Neurod4 influences neurogenesis, neurite outgrowth, and synaptic program initiation. Dysregulated activity of neurogenic bHLH factors is broadly relevant to neurodevelopmental mechanisms and to models that examine altered neuronal differentiation states.
MATH-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Neurod4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Neurod4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Neurod4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MATH-3 protein expression.
This CRISPR knockout system enables efficient generation of Neurod4-deficient cell models for investigation of MATH-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Neurod4 exon(s) critical for MATH-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Neurod4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MATH-3 CRISPR/Cas9 KO Plasmid (m) and MATH-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Neurod4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MATH-3 HDR Plasmid (m) and MATH-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Neurod4 homology arms to support homology-directed repair at defined Neurod4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.