Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAD2B Double Nickase Plasmid (h2): sc-404007-NIC-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAD2B Double Nickase Plasmid (h2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MAD2B Double Nickase Plasmid (h2) and MAD2B Double Nickase Plasmid (h22) encode distinct paired gRNA designs targeting MAD2L2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAD2B Antibody (F-12): sc-377367
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAD2B Double Nickase Plasmid (h2) | sc-404007-NIC-2 | 20 µg | $410.00 |
Human MAD2L2 encodes MAD2B (also known as REV7), a HORMA domain protein that regulates genome stability by participating in translesion DNA synthesis through DNA polymerase ζ and coordinating DNA damage tolerance during replication stress. MAD2B also functions within the Shieldin complex to influence pathway choice in double-strand break repair, promoting non-homologous end joining and limiting DNA end resection, thereby shaping cellular responses to genotoxic insults. Through these roles, MAD2L2 impacts checkpoint control, replication-associated repair, and chromatin-associated repair factor recruitment, processes frequently perturbed in cancer and other disorders linked to defective DNA repair. MAD2L2/MAD2B research tools support mechanistic studies of mutagenesis, synthetic-lethal interaction mapping, and functional interrogation of repair pathway bias in gene editing and genome maintenance assays.
MAD2B Double Nickase Plasmid (h2) consists of a matched pair of plasmids engineered for high-specificity editing of the MAD2L2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAD2L2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAD2L2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAD2L2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.