Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LOXL4 CRISPR/Cas9 KO Plasmid (m): sc-426632
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOXL4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOXL4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LOXL4 Antibody (B-6): sc-365822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOXL4 CRISPR/Cas9 KO Plasmid (m) | sc-426632 | 20 µg | $397.00 |
Overview
Loxl4 encodes lysyl oxidase-like 4 (LOXL4), a copper-dependent amine oxidase that catalyzes oxidative deamination of lysine residues in collagen and elastin, promoting covalent crosslinking and stabilization of the extracellular matrix (ECM). Through ECM remodeling, LOXL4 influences cell adhesion, migration, and mechanotransduction programs connected to tissue development and repair. Altered LOXL family activity has been associated with fibrotic remodeling and changes in tumor microenvironment architecture, making Loxl4 relevant for studying ECM-driven regulation of inflammation, vascular biology, and epithelial–mesenchymal dynamics. In mouse systems, Loxl4 perturbation supports mechanistic work on connective tissue homeostasis and matrix-dependent signaling pathways.
LOXL4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Loxl4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Loxl4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Loxl4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOXL4 protein expression.
This CRISPR knockout system enables efficient generation of Loxl4-deficient cell models for investigation of LOXL4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Loxl4 exon(s) critical for LOXL4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Loxl4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOXL4 CRISPR/Cas9 KO Plasmid (m) and LOXL4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Loxl4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOXL4 HDR Plasmid (m) and LOXL4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Loxl4 homology arms to support homology-directed repair at defined Loxl4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.