LOC147645 CRISPR/Cas9 KO Plasmid (h): sc-406583
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LOC147645 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LOC147645 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LOC147645 CRISPR/Cas9 KO Plasmid (h) | sc-406583 | 20 µg | $397.00 |
Overview
VSIG10L (LOC147645) encodes a predicted V-set and immunoglobulin domain–containing protein that is expected to localize to the cell surface and participate in cell–cell or cell–matrix interactions. Based on domain architecture and homology to other immunoglobulin superfamily members, VSIG10L is implicated in epithelial adhesion programs and regulation of tissue architecture, processes that intersect with differentiation, barrier function, and migratory signaling networks. Variation in expression of related Ig domain proteins is frequently linked to altered tumor microenvironment interactions and metastatic traits, making VSIG10L a candidate target for mechanistic studies in cancer biology and epithelial remodeling. Functional interrogation of LOC147645 can therefore inform pathways governing adhesion-dependent signaling and cellular phenotypic plasticity.
LOC147645 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VSIG10L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VSIG10L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VSIG10L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LOC147645 protein expression.
This CRISPR knockout system enables efficient generation of VSIG10L-deficient cell models for investigation of LOC147645 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VSIG10L exon(s) critical for LOC147645 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VSIG10L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LOC147645 CRISPR/Cas9 KO Plasmid (h) and LOC147645 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VSIG10L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LOC147645 HDR Plasmid (h) and LOC147645 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VSIG10L homology arms to support homology-directed repair at defined VSIG10L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.