LMP2 Antibody (F-10): sc-514345

LMP2 Antibody (F-10) is a mouse monoclonal IgG3 antibody that detects LMP2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LMP2 is a crucial component of the proteasome, a multicatalytic proteinase complex that plays a vital role in the regulated degradation of ubiquitinated proteins, thereby maintaining cellular homeostasis and regulating various cellular processes, including the cell cycle and apoptosis. The proteasome is located in both the nucleus and cytoplasm, where proteasome assembles into a highly ordered ring-shaped structure composed of at least 15 nonidentical subunits. This structural organization is essential for proteasome function, as proteasome allows for efficient processing of proteins into peptides that can be presented by major histocompatibility complex (MHC) molecules, thus playing a significant role in immune response. Upon stimulation with interferon-gamma, LMP2, along with LMP7, replaces housekeeping subunits in the proteasome, enhancing proteasome ability to activate cytotoxic T cells. LMP2 expression is regulated by alternative splicing, resulting in two forms, LMP2.l and LMP2.s, which are produced in lymphoblastoid cell lines and fibroblasts following interferon-gamma stimulation. Anti-LMP2 antibody (F-10) is an invaluable tool for researchers studying the proteasome′s role in cellular regulation and immune response, providing insights into the mechanisms of protein degradation and implications in various diseases.

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LMP2 Antibody (F-10) References:

1. [The relationship between polymorphism of LMP2 and LMP7 genes and the phenotype of ankylosing spondylitis].  |  Ding, H., et al. 1999. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 16: 242-5. PMID: 10431052
2. Autoimmune reactivity against the 20S-proteasome includes immunosubunits LMP2 (beta1i), MECL1 (beta2i) and LMP7 (beta5i).  |  Scheffler, S., et al. 2008. Rheumatology (Oxford). 47: 622-6. PMID: 18375405
3. [Dendritic cells transfected with recombinant adenovirus Ad5F35-LMP2 induces LMP2 specific immunity mediated by cytotoxic T lymphocytes in vitro].  |  Mo, WN., et al. 2008. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 22: 254-6. PMID: 19105335
4. Specific cellular immune responses in mice immunized with DNA, adeno-associated virus and adenoviral vaccines of Epstein-Barr virus-LMP2 alone or in combination.  |  Wang, Z., et al. 2011. Sci China Life Sci. 54: 263-6. PMID: 21416326
5. Nitric oxide-dependent CYP2B degradation is potentiated by a cytokine-regulated pathway and utilizes the immunoproteasome subunit LMP2.  |  Sun, H., et al. 2012. Biochem J. 445: 377-82. PMID: 22612225
6. Association of LMP2 and LMP7 genes within the major histocompatibility complex with insulin-dependent diabetes mellitus: population and family studies.  |  Deng, GY., et al. 1995. Am J Hum Genet. 56: 528-34. PMID: 7847389
7. PRE3, highly homologous to the human major histocompatibility complex-linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl-peptide hydrolyzing activity.  |  Enenkel, C., et al. 1994. FEBS Lett. 341: 193-6. PMID: 7907993
8. Replacement of proteasome subunits X and Y by LMP7 and LMP2 induced by interferon-gamma for acquirement of the functional diversity responsible for antigen processing.  |  Akiyama, K., et al. 1994. FEBS Lett. 343: 85-8. PMID: 8163024
9. Markedly decreased expression of TAP1 and LMP2 genes in HLA class I-deficient human tumor cell lines.  |  Singal, DP., et al. 1996. Immunol Lett. 50: 149-54. PMID: 8803612
10. Development and characterization of mouse anti-human LMP2, LMP7, TAP1 and TAP2 monoclonal antibodies.  |  Kishore, R., et al. 1998. Tissue Antigens. 51: 129-40. PMID: 9510369

The preferred LMP2 antibody is LMP2 Antibody (G-2): sc-373971.