LHX2 Double Nickase Plasmid (h): sc-401071-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LHX2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LHX2 Double Nickase Plasmid (h) and LHX2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LHX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LHX2 Antibody (LHX2A12G1): sc-81311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX2 Double Nickase Plasmid (h) | sc-401071-NIC | 20 µg | $410.00 | |||
LHX2 Double Nickase Plasmid (h2) | sc-401071-NIC-2 | 20 µg | $410.00 |
LHX2 encodes a LIM homeobox transcription factor that regulates lineage specification, progenitor maintenance, and tissue patterning during development. LHX2 functions through DNA binding and LIM domain–mediated protein interactions to coordinate transcriptional programs involved in chromatin regulation, cell fate decisions, and differentiation across neural, ocular, and hematopoietic contexts. In adult tissues, altered LHX2 expression has been associated with dysregulated developmental gene networks and aberrant proliferation or differentiation states. As a context-dependent regulator, LHX2 is frequently studied for its roles in stem cell biology, neurodevelopmental pathways, and transcriptional control mechanisms relevant to disease-associated gene expression signatures.
LHX2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LHX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LHX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LHX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LHX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.