Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LC3B Double Nickase Plasmid (h): sc-417828-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LC3B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LC3B Double Nickase Plasmid (h) and LC3B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAP1LC3B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LC3B Double Nickase Plasmid (h) | sc-417828-NIC | 20 µg | $410.00 | |||
LC3B Double Nickase Plasmid (h2) | sc-417828-NIC-2 | 20 µg | $410.00 |
MAP1LC3B encodes microtubule-associated proteins 1A/1B light chain 3B (LC3B), a core component of the autophagy machinery that undergoes ATG7/ATG3-dependent lipidation to form LC3-II on autophagosome membranes. LC3B supports phagophore elongation and cargo capture via LC3-interacting region (LIR) motifs in selective autophagy receptors such as p62/SQSTM1, linking ubiquitinated substrates to autophagosome formation. Through these interactions, LC3B integrates nutrient-sensing and stress-response pathways, including mTOR and AMPK signaling, to regulate proteostasis and organelle quality control. Dysregulated LC3B-associated autophagy has been implicated in neurodegeneration, infection biology, metabolic dysfunction, and cancer-associated stress adaptation, making it a frequently used marker and mechanistic node in autophagy research.
LC3B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAP1LC3B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAP1LC3B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAP1LC3B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAP1LC3B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.