Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
KiSS-1 Double Nickase Plasmid (h): sc-402710-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KiSS-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KiSS-1 Double Nickase Plasmid (h) and KiSS-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KISS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KiSS-1 Antibody (24-Q): sc-101246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KiSS-1 Double Nickase Plasmid (h) | sc-402710-NIC | 20 µg | $410.00 | |||
KiSS-1 Double Nickase Plasmid (h2) | sc-402710-NIC-2 | 20 µg | $410.00 |
KISS1 encodes KiSS-1, a secreted precursor protein processed into kisspeptins that signal primarily through the GPR54/KISS1R axis to regulate cell migration, invasion, and endocrine signaling. KiSS-1 activity intersects with GPCR-driven pathways including PLC/PKC, intracellular calcium flux, and MAPK signaling, shaping transcriptional programs linked to metastatic potential and reproductive neuroendocrine control. Altered KISS1 expression has been associated with tumor progression and metastatic dissemination in multiple cancer contexts, and with dysregulation of the hypothalamic–pituitary–gonadal axis in reproductive disorders. These features make KISS1 a useful node for studying mechanisms of metastasis suppression, cell–cell communication, and hormone-regulated gene networks in human cells.
KiSS-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KISS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KISS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KISS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KISS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.