KIAA1432 CRISPR/Cas9 KO Plasmid (h): sc-417074
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA1432 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIAA1432 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA1432 CRISPR/Cas9 KO Plasmid (h) | sc-417074 | 20 µg | $397.00 |
Overview
RIC1 (KIAA1432) encodes a conserved component of the RIC1–RGP1 guanine nucleotide exchange factor complex that regulates Rab6-dependent membrane trafficking. This complex supports tethering and retrograde transport from endosomes to the trans-Golgi network and contributes to Golgi organization, vesicle docking, and polarized secretion. Through its role in maintaining Golgi integrity and intracellular transport fidelity, KIAA1432 is relevant to cellular phenotypes linked to migration, adhesion, and proteostasis. Dysregulated vesicular trafficking and Golgi-associated pathways are frequently implicated in proliferative and neurodevelopmental disease biology, making RIC1 a useful target for mechanistic studies of trafficking-dependent signaling.
KIAA1432 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RIC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RIC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RIC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIAA1432 protein expression.
This CRISPR knockout system enables efficient generation of RIC1-deficient cell models for investigation of KIAA1432 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RIC1 exon(s) critical for KIAA1432 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RIC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIAA1432 CRISPR/Cas9 KO Plasmid (h) and KIAA1432 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RIC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIAA1432 HDR Plasmid (h) and KIAA1432 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RIC1 homology arms to support homology-directed repair at defined RIC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.