Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
involucrin Double Nickase Plasmid (h): sc-416724-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- involucrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- involucrin Double Nickase Plasmid (h) and involucrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IVL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: involucrin Antibody (SY5): sc-21748
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
involucrin Double Nickase Plasmid (h) | sc-416724-NIC | 20 µg | $410.00 | |||
involucrin Double Nickase Plasmid (h2) | sc-416724-NIC-2 | 20 µg | $410.00 |
IVL encodes involucrin, a keratinocyte differentiation marker that becomes crosslinked into the cornified envelope by transglutaminases to strengthen the epidermal barrier. Involucrin participates in terminal differentiation programs coordinated with keratin intermediate filament remodeling and calcium-dependent signaling that supports cornification. Altered IVL expression and envelope assembly have been associated with dysregulated epidermal homeostasis in inflammatory and hyperproliferative skin conditions, including psoriasis and atopic dermatitis, and are frequently examined in the context of barrier defects. As a result, IVL is widely used to study stratified epithelium maturation, barrier formation, and transcriptional networks governing keratinocyte fate.
involucrin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IVL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IVL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IVL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IVL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.