Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ILKAP CRISPR/Cas9 KO Plasmid (h): sc-405879
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ILKAP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ILKAP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ILKAP Antibody (41): sc-136341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ILKAP CRISPR/Cas9 KO Plasmid (h) | sc-405879 | 20 µg | $397.00 |
Overview
ILKAP (integrin-linked kinase–associated serine/threonine phosphatase 2C) encodes a PP2C-family phosphatase that associates with integrin-linked signaling complexes and modulates phosphorylation-dependent control of cell adhesion and cytoskeletal organization. By dephosphorylating specific substrates within integrin/ECM-associated pathways, ILKAP can influence cellular migration, mechanotransduction, and growth factor–responsive signaling outputs. Altered ILKAP expression or activity has been studied in contexts involving dysregulated adhesion signaling, stress responses, and pathway rewiring that supports malignant phenotypes. As a result, ILKAP is frequently interrogated in models of tumor cell invasion, survival signaling, and adhesion-dependent regulation of proliferation.
ILKAP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ILKAP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ILKAP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ILKAP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ILKAP protein expression.
This CRISPR knockout system enables efficient generation of ILKAP-deficient cell models for investigation of ILKAP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ILKAP exon(s) critical for ILKAP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ILKAP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ILKAP CRISPR/Cas9 KO Plasmid (h) and ILKAP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ILKAP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ILKAP HDR Plasmid (h) and ILKAP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ILKAP homology arms to support homology-directed repair at defined ILKAP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.