IL-4I1 CRISPR/Cas9 KO Plasmid (m): sc-420354
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-4I1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-4I1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-4I1 CRISPR/Cas9 KO Plasmid (m) | sc-420354 | 20 µg | $397.00 |
Overview
Il4i1 encodes interleukin-4–induced gene 1 (IL-4I1), a secreted L-amino-acid oxidase expressed by antigen-presenting cells and inducible in inflammatory and tumor-like microenvironments. IL-4I1 catalyzes oxidative deamination of amino acids such as phenylalanine, generating metabolic products including hydrogen peroxide that can shape redox balance and immune signaling. Through amino-acid metabolism and oxidative stress pathways, IL-4I1 influences myeloid cell programs, T cell activation thresholds, and broader immunoregulatory circuits. Dysregulated IL-4I1 activity has been linked in the literature to altered immune suppression, chronic inflammation, and cancer-associated immune evasion, making it a useful node for mechanistic immunometabolism studies in mouse systems.
IL-4I1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il4i1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il4i1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il4i1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-4I1 protein expression.
This CRISPR knockout system enables efficient generation of Il4i1-deficient cell models for investigation of IL-4I1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il4i1 exon(s) critical for IL-4I1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il4i1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-4I1 CRISPR/Cas9 KO Plasmid (m) and IL-4I1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il4i1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-4I1 HDR Plasmid (m) and IL-4I1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il4i1 homology arms to support homology-directed repair at defined Il4i1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.