IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h): sc-401045-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h) and IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSF2RB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-3/IL-5/GM-CSFRβ Antibody (1C1): sc-21765
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h) | sc-401045-NIC | 20 µg | $410.00 | |||
IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h2) | sc-401045-NIC-2 | 20 µg | $410.00 |
CSF2RB encodes the common beta chain (βc) shared by the human IL-3, IL-5, and GM-CSF receptors, forming high-affinity signaling complexes that regulate hematopoietic progenitor proliferation, survival, and differentiation. Upon cytokine engagement, βc-dependent signaling activates JAK2/STAT5, PI3K–AKT, and RAS–MAPK pathways, coordinating myeloid lineage commitment and inflammatory effector functions. CSF2RB activity shapes granulocyte and macrophage responses and influences cytokine-driven cell-state transitions in immune and hematologic systems. Dysregulated βc signaling has been associated with aberrant myeloid activation and inflammatory programs, making CSF2RB a key node for mechanistic studies of cytokine receptor signaling.
IL-3/IL-5/GM-CSFRβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSF2RB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSF2RB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSF2RB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSF2RB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.