Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-2Rγ Double Nickase Plasmid (h): sc-401876-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-2Rγ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-2Rγ Double Nickase Plasmid (h) and IL-2Rγ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IL2RG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-2Rγ Antibody (A-10): sc-271060
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-2Rγ Double Nickase Plasmid (h) | sc-401876-NIC | 20 µg | $410.00 | |||
IL-2Rγ Double Nickase Plasmid (h2) | sc-401876-NIC-2 | 20 µg | $410.00 |
IL2RG encodes the common gamma chain (IL-2Rγ, CD132), an essential signaling subunit shared by multiple cytokine receptors including those for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Upon cytokine binding, IL-2Rγ couples receptor complexes to JAK3 and downstream STAT signaling, coordinating lymphocyte development, survival, and effector differentiation across T cells, B cells, and NK cells. This axis integrates with broader immune regulatory programs such as cytokine-driven proliferation, differentiation, and homeostatic maintenance. Disruption of IL2RG-dependent signaling is strongly linked to severe defects in adaptive and innate immune cell function, making IL2RG a key gene for mechanistic studies of immunodeficiency and cytokine receptor signaling.
IL-2Rγ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IL2RG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IL2RG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IL2RG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IL2RG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.