IL-12 p70 Antibody (14L7): sc-74150

IL-12 p70 Antibody (14L7) is a mouse monoclonal IgG1 antibody that detects IL-12 p70 in human samples through applications such as immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IL-12 p70 is a crucial cytokine that plays a significant role in the immune response by promoting the differentiation of naive CD4(+) T cells into type 1 helper T cells, which are essential for the production of interferon-γ (IFN-γ). This process is vital for enhancing the immune system′s ability to combat infections and tumors, as IFN-γ activates macrophages and natural killer cells, leading to increased production of tumor necrosis factor α (TNF-α). Furthermore, IL-12 p70 is composed of two subunits, p35 and p40, which are regulated in a cell type-specific manner, allowing for precise control of immune responses. The ability of IL-12 p70 to inhibit IL-4 mediated suppression of IFN-γ production underscores its importance in maintaining a robust immune response. Additionally, IL-12 p70 exhibits anti-angiogenic properties, as IL-12 p70 stimulates the production of inducible protein-10 (IP-10) in response to IFN-γ, further highlighting its multifaceted role in immune regulation and potential therapeutic applications. Anti-IL-12 p70 antibody (14L7) is an invaluable tool for researchers studying the intricate dynamics of immune responses and the therapeutic potential of cytokines in various diseases.

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IL-12 p70 Antibody (14L7) References:

1. CD40 engagement stimulates IL-12 p70 production by human microglial cells: basis for Th1 polarization in the CNS.  |  Becher, B., et al. 2000. J Neuroimmunol. 102: 44-50. PMID: 10626665
2. Total serum IL-12 and IL-12p40, but not IL-12p70, are increased in the serum of older subjects; relationship to CD3(+)and NK subsets.  |  Rea, IM., et al. 2000. Cytokine. 12: 156-9. PMID: 10671301
3. Following direct CD40 activation, human primary microglial cells produce IL-12 p40 but not bioactive IL-12 p70.  |  de Goër de Herve, MG., et al. 2001. Cytokine. 14: 88-96. PMID: 11356009
4. IL-12/IL-18-dependent IFN-gamma release by murine dendritic cells.  |  Stober, D., et al. 2001. J Immunol. 167: 957-65. PMID: 11441104
5. Smokeless tobacco extract decreases IL-12 production from LPS-stimulated but increases IL-12 from IFN-gamma-stimulated macrophages.  |  Petro, TM., et al. 2002. Int Immunopharmacol. 2: 345-55. PMID: 11811937
6. Increased circulating interleukin-12 (IL-12) p40 in pulmonary sarcoidosis.  |  Shigehara, K., et al. 2003. Clin Exp Immunol. 132: 152-7. PMID: 12653850
7. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.  |  Spensieri, F., et al. 2006. Infect Immun. 74: 2831-8. PMID: 16622221
8. IL-12 p40 homodimer, the so-called biologically inactive molecule, induces nitric oxide synthase in microglia via IL-12R beta 1.  |  Jana, M., et al. 2009. Glia. 57: 1553-65. PMID: 19306359
9. Notch- and transducin-like enhancer of split (TLE)-dependent histone deacetylation explain interleukin 12 (IL-12) p70 inhibition by zymosan.  |  Alvarez, Y., et al. 2011. J Biol Chem. 286: 16583-95. PMID: 21402701
10. Mammalian target of rapamycin complex 2 (mTORC2) regulates LPS-induced expression of IL-12 and IL-23 in human dendritic cells.  |  Wei, WC., et al. 2015. J Leukoc Biol. 97: 1071-80. PMID: 25877925