IKK alpha Double Nickase Plasmid (h): sc-400492-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IKK alpha Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IKK alpha Double Nickase Plasmid (h) and IKK alpha Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHUK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IKK alpha Antibody (B-8): sc-7606
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IKK alpha Double Nickase Plasmid (h) | sc-400492-NIC | 20 µg | $410.00 | |||
IKK alpha Double Nickase Plasmid (h2) | sc-400492-NIC-2 | 20 µg | $410.00 |
CHUK encodes IKK alpha (IKKα), a catalytic subunit of the IκB kinase complex that regulates NF-κB signaling through phosphorylation-dependent control of IκB stability and downstream transcriptional programs. Beyond canonical NF-κB activation, IKKα plays a prominent role in noncanonical NF-κB signaling via processing of NF-κB2 p100 to p52, influencing lymphoid organogenesis, B cell maturation, and inflammatory gene expression. IKKα also interfaces with pathways governing cell survival, differentiation, and stress responses, linking CHUK activity to context-dependent regulation of proliferation and apoptosis. Dysregulated IKKα/NF-κB axis activity is frequently studied in inflammation-associated tumor biology, immune dysregulation, and chronic inflammatory disease mechanisms.
IKK alpha Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHUK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHUK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHUK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHUK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.