Id1 Double Nickase Plasmid (h): sc-400341-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Id1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Id1 Double Nickase Plasmid (h) and Id1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ID1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Id1 Antibody (B-8): sc-133104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Id1 Double Nickase Plasmid (h) | sc-400341-NIC | 20 µg | $410.00 | |||
Id1 Double Nickase Plasmid (h2) | sc-400341-NIC-2 | 20 µg | $410.00 |
Human ID1 encodes Id1, a helix–loop–helix (HLH) protein that lacks a DNA-binding basic region and functions as a dominant-negative regulator of bHLH transcription factors. By sequestering E-proteins, Id1 modulates transcriptional programs controlling cell-cycle progression, lineage commitment, and differentiation, and it is frequently linked to stem-like states and cellular plasticity. ID1 expression is regulated downstream of mitogenic and developmental cues, including BMP/TGF-β-associated signaling, and integrates with pathways that influence proliferation and migration. Dysregulated ID1 activity has been associated with oncogenic transcriptional networks and altered differentiation in multiple disease contexts, supporting its use as a target for mechanistic studies of growth control and fate decisions.
Id1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ID1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ID1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ID1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ID1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.