HUNK Antibody (B-7): sc-514689

HUNK Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HUNK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HUNK (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HUNK protein, also known as hormonally upregulated Neu-associated kinase or MAK-V in mice, is a novel SNF1-related serine/threonine kinase that plays a crucial role in cellular signaling pathways. HUNK protein is primarily localized in the cytoplasm and centrosome, which is significant because this localization is essential for regulating cell cycle progression and cellular responses to hormonal signals. Notably, overexpression of HUNK protein has been linked to approximately 50% of breast carcinomas, suggesting potential as a diagnostic and prognostic molecular marker in cancer. Additionally, HUNK protein interacts with serine/threonine-protein kinase SNF1-like kinase 2 (SIK), which phosphorylates insulin receptor substrate 1 (IRS1) at Ser 794 in insulin-stimulated adipocytes, thereby modulating insulin signal transduction efficiency. SIK activation is further regulated by phosphorylation on Thr 175 by STK11 in conjunction with STE20-related adapter-alpha and CAB39, highlighting the intricate network of signaling pathways in which HUNK is involved.

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HUNK Antibody (B-7) References:

1. Cloning and characterization of Hunk, a novel mammalian SNF1-related protein kinase.  |  Gardner, HP., et al. 2000. Genomics. 63: 46-59. PMID: 10662544
2. Developmental role of the SNF1-related kinase Hunk in pregnancy-induced changes in the mammary gland.  |  Gardner, HP., et al. 2000. Development. 127: 4493-509. PMID: 11003847
3. The MAK-V protein kinase regulates endocytosis in mouse.  |  Korobko, IV., et al. 2000. Mol Gen Genet. 264: 411-8. PMID: 11129044
4. Subcellular localization of MAK-V/Hunk protein kinase expressed in COS-1 cells.  |  Korobko, EV., et al. 2004. Cell Biol Int. 28: 49-56. PMID: 14759768
5. [Proteinkinase MAK-V/Hunk as a possible dianostic and prognostic marker of human breast carcinoma].  |  Korobko, IV., et al. 2004. Arkh Patol. 66: 6-9. PMID: 15575376
6. [Molecular cloning and characterization of the mouse mak-v/Hunk gene promoter].  |  Korobko, EV., et al. 2005. Mol Biol (Mosk). 39: 72-9. PMID: 15773550
7. HUNK phosphorylates EGFR to regulate breast cancer metastasis.  |  Williams, CB., et al. 2020. Oncogene. 39: 1112-1124. PMID: 31597954
8. HUNK Signaling in Metastatic Breast Cancer.  |  Dilday, T., et al. 2020. Oncoscience. 7: 30-33. PMID: 32676513
9. HUNK Gene Alterations in Breast Cancer.  |  Ramos-Solis, N., et al. 2022. Biomedicines. 10: PMID: 36551828
10. HUNK inhibits epithelial-mesenchymal transition of CRC via direct phosphorylation of GEF-H1 and activating RhoA/LIMK-1/CFL-1.  |  Han, X., et al. 2023. Cell Death Dis. 14: 327. PMID: 37193711
11. Hypoxia inhibits HUNK kinase activity to induce epithelial-mesenchymal transition.  |  Jiang, S., et al. 2023. Biochem Biophys Res Commun. 681: 271-275. PMID: 37793312
12. HUNK inhibits cargo uptake and lysosomal traffic in the caveolar pathway via the AGAP3/ARF6.  |  Jiang, S., et al. 2024. Sci Bull (Beijing). 69: 173-178. PMID: 38071109
13. HUNK as a key regulator of tumor-associated macrophages in triple negative breast cancer.  |  Ramos Solis, N., et al. 2024. Oncoimmunology. 13: 2364382. PMID: 38846083

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HUNK Antibody (B-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HUNK Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HUNK Antibody (B-7) Specifications

Alternate Product Name: HUNK

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 361-514

Epitope Target: HUNK

Epitope Species: human

Product Reactivity/Detection: HUNK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HUNK Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin