Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HPK1 Double Nickase Plasmid (h): sc-402367-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HPK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HPK1 Double Nickase Plasmid (h) and HPK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAP4K1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HPK1 Antibody (G-9): sc-374183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPK1 Double Nickase Plasmid (h) | sc-402367-NIC | 20 µg | $410.00 | |||
HPK1 Double Nickase Plasmid (h2) | sc-402367-NIC-2 | 20 µg | $410.00 |
MAP4K1 encodes hematopoietic progenitor kinase 1 (HPK1), a Ste20 family serine/threonine kinase that functions as an upstream regulator of MAPK signaling in immune cells. HPK1 transduces signals downstream of antigen receptors and costimulatory pathways, shaping activation thresholds through modulation of ERK/JNK cascades, NF-κB-linked signaling nodes, and cytoskeletal remodeling. By tuning phosphorylation-dependent adaptor and ubiquitin-network interactions, HPK1 influences T cell receptor signaling strength, cytokine production programs, and immune synapse dynamics. Altered MAP4K1/HPK1 activity has been associated with dysregulated immune signaling states and is frequently studied in immuno-oncology and inflammation-focused molecular pathways.
HPK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAP4K1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAP4K1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAP4K1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAP4K1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.