HN1 Double Nickase Plasmid (h): sc-412133-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HN1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HN1 Double Nickase Plasmid (h) and HN1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HN1 Antibody (3G6): sc-517032
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HN1 Double Nickase Plasmid (h) | sc-412133-NIC | 20 µg | $410.00 | |||
HN1 Double Nickase Plasmid (h2) | sc-412133-NIC-2 | 20 µg | $410.00 |
HN1 (hematological and neurological expressed 1) encodes a small, conserved protein implicated in regulation of cell proliferation and differentiation programs, with dynamic expression during development and in regenerating tissues. In human cells, HN1 abundance has been linked to cell-cycle control and stress-adaptive transcriptional states, and it is frequently studied in the context of signaling networks that influence growth and lineage plasticity. Altered HN1 expression has been reported across multiple tumor types, where it is used as a molecular correlate of aggressive phenotypes and remodeling of proliferative pathways. These features make HN1 a useful target for mechanistic studies of growth control, phenotypic switching, and context-dependent gene regulation.
HN1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.