Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HB-EGF Double Nickase Plasmid (m): sc-420827-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HB-EGF Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HB-EGF Double Nickase Plasmid (m) and HB-EGF Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hbegf. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HB-EGF Antibody (H-1): sc-365182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HB-EGF Double Nickase Plasmid (m) | sc-420827-NIC | 20 µg | $410.00 |
Mouse Hbegf encodes heparin-binding EGF-like growth factor (HB-EGF), a membrane-anchored EGFR/ERBB ligand that is proteolytically shed to generate a soluble mitogen. HB-EGF signaling activates canonical receptor tyrosine kinase pathways including MAPK/ERK and PI3K/AKT, shaping epithelial and stromal proliferation, survival, migration, and wound-response programs. Through regulated ectodomain shedding and autocrine/paracrine signaling, HB-EGF contributes to tissue remodeling and cell-state transitions such as mesenchymal-like motility. Dysregulated HB-EGF–EGFR axis activity is frequently studied in contexts of hyperproliferation, inflammation-associated remodeling, and oncogenic signaling networks.
HB-EGF Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hbegf locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hbegf. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hbegf function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hbegf-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.