Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HB-EGF Double Nickase Plasmid (h): sc-400795-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HB-EGF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HB-EGF Double Nickase Plasmid (h) and HB-EGF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HBEGF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HB-EGF Antibody (G-11): sc-74441
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HB-EGF Double Nickase Plasmid (h) | sc-400795-NIC | 20 µg | $410.00 | |||
HB-EGF Double Nickase Plasmid (h2) | sc-400795-NIC-2 | 20 µg | $410.00 |
Human HBEGF encodes heparin-binding EGF-like growth factor (HB-EGF), a membrane-anchored ligand that can be proteolytically shed to generate a soluble growth factor. HB-EGF primarily signals through EGFR/ERBB family receptors to regulate MAPK/ERK and PI3K–AKT pathway activity, influencing cell proliferation, survival, migration, and wound-associated remodeling. Its expression and ectodomain shedding are tightly linked to inflammatory and stress-responsive signaling and to ADAM-mediated protease activity at the cell surface. Dysregulated HBEGF/EGFR signaling has been associated with altered epithelial and vascular responses in contexts such as fibrosis, cancer biology, and cardiovascular remodeling, making it relevant for mechanistic studies of growth factor–driven programs.
HB-EGF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HBEGF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HBEGF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HBEGF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HBEGF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.