Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HAUSP Double Nickase Plasmid (h): sc-402013-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HAUSP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HAUSP Double Nickase Plasmid (h) and HAUSP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HAUSP Antibody (H-12): sc-137008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAUSP Double Nickase Plasmid (h) | sc-402013-NIC | 20 µg | $410.00 | |||
HAUSP Double Nickase Plasmid (h2) | sc-402013-NIC-2 | 20 µg | $410.00 |
USP7 encodes the deubiquitinating enzyme HAUSP, a ubiquitin-specific protease that removes ubiquitin from key regulatory proteins to control their stability, localization, and activity. HAUSP is central to ubiquitin-dependent proteostasis and participates in DNA damage response, cell-cycle regulation, and chromatin-associated processes by modulating factors such as p53 and MDM2. Through these interactions, USP7 influences checkpoint signaling, replication stress handling, and transcriptional programs that maintain genome integrity. Dysregulated USP7 activity has been linked to aberrant ubiquitin signaling observed in multiple disease-relevant contexts, including oncogenic pathways and neurodevelopmental phenotypes, making it a frequent target for mechanistic studies.
HAUSP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.