GSG1 CRISPR/Cas9 KO Plasmid (m): sc-420702
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GSG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSG1 CRISPR/Cas9 KO Plasmid (m) | sc-420702 | 20 µg | $397.00 |
Overview
Mouse Gsg1 encodes GSG1, a germ cell–associated membrane protein enriched in the testis and linked to late stages of spermatogenesis. Reported biology supports roles in male germ cell differentiation and sperm maturation, implicating cellular processes such as membrane organization, protein trafficking, and spermiogenic remodeling. Because proper expression of spermatogenesis genes is essential for fertility, perturbation of Gsg1 is relevant to studies of male reproductive phenotypes, including infertility-associated mechanisms and testis-specific gene regulation.
GSG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gsg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gsg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gsg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GSG1 protein expression.
This CRISPR knockout system enables efficient generation of Gsg1-deficient cell models for investigation of GSG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gsg1 exon(s) critical for GSG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gsg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GSG1 CRISPR/Cas9 KO Plasmid (m) and GSG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gsg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GSG1 HDR Plasmid (m) and GSG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gsg1 homology arms to support homology-directed repair at defined Gsg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.