GRP Double Nickase Plasmid (h): sc-402811-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRP Double Nickase Plasmid (h) and GRP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRP Antibody (E-11): sc-271045
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRP Double Nickase Plasmid (h) | sc-402811-NIC | 20 µg | $410.00 | |||
GRP Double Nickase Plasmid (h2) | sc-402811-NIC-2 | 20 µg | $410.00 |
Gastrin-releasing peptide (GRP) is a secreted neuropeptide that signals primarily through the GRP receptor (GRPR), a GPCR that activates phospholipase C–IP3/DAG–PKC signaling, mobilizes intracellular Ca2+, and engages MAPK/ERK and PI3K/AKT pathways to modulate cell excitability, secretion, and proliferation. In the nervous system and peripheral neuroendocrine tissues, GRP contributes to neurotransmission, circadian and stress-related responses, and regulation of smooth muscle and gastrointestinal function. Dysregulated GRP–GRPR signaling has been implicated in neuroendocrine differentiation and mitogenic programs in several tumor contexts, and it is also studied in sensory circuits involved in itch and pain. These attributes make GRP a useful node for dissecting peptide-mediated autocrine/paracrine signaling, GPCR pathway crosstalk, and stimulus-dependent transcriptional responses.
GRP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.