granzyme K Antibody (GM6C3): sc-56125

granzyme K Antibody (GM6C3) is a mouse monoclonal IgG2b kappa light chain antibody that detects human granzyme K by flow cytometry (FCM). anti-granzyme K antibody (GM6C3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Granzyme K, also known as GMZK or NK-tryptase-2, is a serine protease that plays a crucial role in the immune response, particularly in the granules of natural killer cells and cytotoxic T lymphocytes. GMZK is primarily expressed in the thymus, lung, spleen, and peripheral blood leukocytes, where GMZK contributes to the lysis of target cells during cell-mediated immune responses. GMZK contains one peptidase S1 domain, which is essential for enzymatic activity, allowing GMZK to cleave specific substrates and induce apoptosis in infected or malignant cells. Understanding granzyme K function is vital, as granzyme K not only aids in the elimination of harmful cells but also provides insights into the mechanisms of immune regulation and potential therapeutic targets for enhancing immune responses in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

granzyme K Antibody (GM6C3) References:

1. Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma.  |  Wilharm, E., et al. 1999. J Biol Chem. 274: 27331-7. PMID: 10480954
2. The 2.2-A crystal structure of human pro-granzyme K reveals a rigid zymogen with unusual features.  |  Hink-Schauer, C., et al. 2002. J Biol Chem. 277: 50923-33. PMID: 12384499
3. Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interactions.  |  Shi, L., et al. 1992. J Exp Med. 176: 1521-9. PMID: 1460416
4. Molecular understanding of oxygen-tension and patient-variability effects on ex vivo expanded T cells.  |  Haddad, H., et al. 2004. Biotechnol Bioeng. 87: 437-50. PMID: 15286980
5. Expression of enzymatically active human granzyme 3 in Escherichia coli for analysis of its substrate specificity.  |  Hirata, Y., et al. 2006. Arch Biochem Biophys. 446: 35-43. PMID: 16405860
6. Characterization of three serine esterases isolated from human IL-2 activated killer cells.  |  Hameed, A., et al. 1988. J Immunol. 141: 3142-7. PMID: 3262682
7. Cloning of cDNA for human granzyme 3.  |  Przetak, MM., et al. 1995. FEBS Lett. 364: 268-71. PMID: 7758581
8. Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3.  |  Sayers, TJ., et al. 1996. J Leukoc Biol. 59: 763-8. PMID: 8656064

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-granzyme K Antibody (GM6C3) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-granzyme K Antibody (GM6C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

granzyme K Antibody (GM6C3) Specifications

Alternate Product Name: granzyme K

Product Clone Name: GM6C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: granzyme K

Epitope Species: human

Product Reactivity/Detection: granzyme K transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for granzyme K Antibody (GM6C3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin