Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
glypican-5 CRISPR/Cas9 KO Plasmid (h): sc-407289
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- glypican-5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the glypican-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: glypican-5 Antibody (F-3): sc-390838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
glypican-5 CRISPR/Cas9 KO Plasmid (h) | sc-407289 | 20 µg | $397.00 |
Overview
Human GPC5 encodes glypican-5, a glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan that localizes to the cell surface and shapes the distribution and signaling potency of secreted morphogens and growth factors. By binding heparan sulfate–interacting ligands, glypican-5 modulates pathways such as Wnt/β-catenin, Hedgehog, and FGF signaling, influencing cell proliferation, differentiation, and tissue patterning. Its expression and genomic alterations have been associated with developmental processes and cancer-related phenotypes, including changes in cell growth and invasiveness. As an extracellular regulator of ligand–receptor interactions, GPC5 is relevant for studies of signal transduction dynamics and microenvironment-dependent signaling.
glypican-5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPC5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPC5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPC5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish glypican-5 protein expression.
This CRISPR knockout system enables efficient generation of GPC5-deficient cell models for investigation of glypican-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPC5 exon(s) critical for glypican-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPC5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by glypican-5 CRISPR/Cas9 KO Plasmid (h) and glypican-5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPC5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by glypican-5 HDR Plasmid (h) and glypican-5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPC5 homology arms to support homology-directed repair at defined GPC5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.