GLTSCR1 Double Nickase Plasmid (h): sc-409913-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLTSCR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GLTSCR1 Double Nickase Plasmid (h) and GLTSCR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLTSCR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GLTSCR1 Antibody (H-10): sc-515086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLTSCR1 Double Nickase Plasmid (h) | sc-409913-NIC | 20 µg | $410.00 | |||
GLTSCR1 Double Nickase Plasmid (h2) | sc-409913-NIC-2 | 20 µg | $410.00 |
GLTSCR1 (glioma tumor suppressor candidate region gene 1) encodes a nuclear protein implicated in the regulation of chromatin-associated processes and transcriptional control, consistent with roles in maintaining proper gene expression programs. Reported interactions link GLTSCR1 to epigenetic regulatory complexes and pathways that influence cell cycle progression, DNA damage responses, and cellular differentiation states. Altered GLTSCR1 expression or genomic perturbation has been associated with tumor-related contexts, particularly in glioma-associated regions, supporting its relevance for studying mechanisms of oncogenic transcriptional rewiring. As a human gene, GLTSCR1 is frequently examined in models that probe nuclear organization, chromatin regulation, and genotype-to-phenotype relationships in proliferative disease biology.
GLTSCR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLTSCR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLTSCR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLTSCR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLTSCR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.