GILZ Double Nickase Plasmid (h): sc-401424-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GILZ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GILZ Double Nickase Plasmid (h) and GILZ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TSC22D3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GILZ Antibody (B-2): sc-515835
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GILZ Double Nickase Plasmid (h) | sc-401424-NIC | 20 µg | $410.00 | |||
GILZ Double Nickase Plasmid (h2) | sc-401424-NIC-2 | 20 µg | $410.00 |
TSC22D3 encodes glucocorticoid-induced leucine zipper (GILZ), an inducible transcriptional regulator that integrates glucocorticoid receptor signaling with inflammatory and stress-response programs. GILZ modulates gene expression by interacting with key transcription factors and signaling nodes, including NF-κB and AP-1, thereby influencing cytokine production, immune-cell activation, and apoptosis. In human cells, TSC22D3 is linked to regulation of T cell responses, macrophage polarization, and epithelial inflammatory signaling, positioning it within pathways relevant to immune homeostasis and tissue remodeling. Dysregulated GILZ expression has been associated with altered inflammatory states and context-dependent changes in cell survival and differentiation that are studied in autoimmune, metabolic, and cancer biology models.
GILZ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TSC22D3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TSC22D3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TSC22D3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TSC22D3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.