G_β Antibody (B-11): sc-166249

Gβ Antibody (B-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects the G beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-G beta antibody (B-11) is available as the non-conjugated form. G beta protein plays a crucial role in the function of heterotrimeric G proteins, which are essential for transmitting signals from various cell surface receptors to intracellular effectors. These receptors detect a wide array of extracellular stimuli, such as hormones and neurotransmitters, while the effectors, including adenyl cyclase, generate intracellular messengers that mediate cellular responses. G protein complex consists of three subunits—alpha, beta, and gamma—each encoded by distinct gene families. β subunits are vital for regulating the activity of G protein alpha subunits and certain signal transduction pathways. Among the five members of the β-subunit family in mammals, Gβ5 stands out due to its unique structure, exhibiting only 50% homology with other β subunits, Gβ1-4, which share at least 83% homology. Gβ5 is predominantly expressed in brain, pancreas, kidney, and heart, highlighting its importance in various physiological processes.

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G_β Antibody (B-11) References:

1. Gbeta gamma isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Galphas and Galphaq.  |  Evanko, DS., et al. 2001. J Biol Chem. 276: 23945-53. PMID: 11294873
2. Group G beta-hemolytic streptococcal bacteremia characterized by 16S ribosomal RNA gene sequencing.  |  Woo, PC., et al. 2001. J Clin Microbiol. 39: 3147-55. PMID: 11526143
3. Ca2+ and phosphatidylinositol 4,5-bisphosphate stabilize a Gbeta gamma-sensitive state of Ca V2 Ca 2+ channels.  |  Rousset, M., et al. 2004. J Biol Chem. 279: 14619-30. PMID: 14722074
4. Ric-8B interacts with G alpha olf and G gamma 13 and co-localizes with G alpha olf, G beta 1 and G gamma 13 in the cilia of olfactory sensory neurons.  |  Kerr, DS., et al. 2008. Mol Cell Neurosci. 38: 341-8. PMID: 18462949
5. G-protein signaling modulator-3 regulates heterotrimeric G-protein dynamics through dual association with Gβ and Gαi protein subunits.  |  Giguère, PM., et al. 2012. J Biol Chem. 287: 4863-74. PMID: 22167191
6. Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.  |  Ismael, A., et al. 2016. Sci Signal. 9: ra38. PMID: 27072657
7. Anti-nociceptive action of peripheral mu-opioid receptors by G-beta-gamma protein-mediated inhibition of TRPM3 channels.  |  Dembla, S., et al. 2017. Elife. 6: PMID: 28826482
8. Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit.  |  Tsai, CJ., et al. 2019. Elife. 8: PMID: 31251171
9. Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers.  |  Kofuji, P., et al. 1995. Proc Natl Acad Sci U S A. 92: 6542-6. PMID: 7604029
10. An eye-specific G beta subunit essential for termination of the phototransduction cascade.  |  Dolph, PJ., et al. 1994. Nature. 370: 59-61. PMID: 8015606

The preferred G_β antibody is G_β Antibody (H-1): sc-166123.