Gastrin CRISPR/Cas9 KO Plasmid (h): sc-403220
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gastrin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gastrin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gastrin Antibody (B-10): sc-28302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gastrin CRISPR/Cas9 KO Plasmid (h) | sc-403220 | 20 µg | $397.00 |
Overview
GAST encodes gastrin, a prohormone processed into bioactive peptides that regulate gastric acid secretion and coordinate mucosal growth and differentiation in the gastrointestinal tract. Through binding to the cholecystokinin B receptor (CCKBR), gastrin activates G protein–coupled signaling networks including PLC/IP3/Ca2+, PKC, and MAPK/ERK pathways, influencing secretory function and epithelial proliferation. Dysregulated gastrin production or signaling is associated with altered gastric physiology and has been studied in the context of hypergastrinemia, gastric neuroendocrine biology, and inflammation-associated remodeling. As a gut hormone axis gene, GAST is a useful entry point for dissecting endocrine–paracrine communication and downstream transcriptional programs in gastric and pancreatic model systems.
Gastrin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GAST gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GAST together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GAST open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gastrin protein expression.
This CRISPR knockout system enables efficient generation of GAST-deficient cell models for investigation of Gastrin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GAST exon(s) critical for Gastrin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GAST genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gastrin CRISPR/Cas9 KO Plasmid (h) and Gastrin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GAST locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gastrin HDR Plasmid (h) and Gastrin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GAST homology arms to support homology-directed repair at defined GAST target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.