folliculin Double Nickase Plasmid (h): sc-403316-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- folliculin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- folliculin Double Nickase Plasmid (h) and folliculin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FLCN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: folliculin Antibody (D-4): sc-271558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
folliculin Double Nickase Plasmid (h) | sc-403316-NIC | 20 µg | $410.00 | |||
folliculin Double Nickase Plasmid (h2) | sc-403316-NIC-2 | 20 µg | $410.00 |
FLCN encodes folliculin, a tumor suppressor–associated protein that coordinates nutrient sensing, lysosomal signaling, and cellular metabolism through interactions with FNIP1/2 and regulation of AMPK–mTORC1 and Rag GTPase pathways. Folliculin contributes to autophagy control, mitochondrial homeostasis, and transcriptional programs linked to TFEB/TFE3, influencing lysosome biogenesis and stress responses. Loss-of-function variants in FLCN are implicated in Birt–Hogg–Dubé syndrome and are associated with renal tumor predisposition and pulmonary cyst formation, making FLCN a key node for studying genotype-to-phenotype mechanisms. Experimental modulation of folliculin supports investigations into energy homeostasis, cell growth control, and epithelial differentiation relevant to disease biology.
folliculin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FLCN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FLCN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FLCN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FLCN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.