FAM55D CRISPR/Cas9 KO Plasmid (h): sc-412343
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM55D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM55D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM55D CRISPR/Cas9 KO Plasmid (h) | sc-412343 | 20 µg | $397.00 |
Overview
NXPE4 encodes the human protein FAM55D, a member of the neurexophilin and PC-esterase domain protein family that is predicted to be secreted or membrane-associated and enriched in neural contexts. Although its molecular function remains incompletely characterized, family features suggest roles in extracellular protein interactions and cell–cell communication processes that can influence neuronal connectivity and synaptic organization. Transcript-level variation and tissue-specific expression patterns have been explored in genomics datasets, supporting continued investigation of NXPE4 in neurobiology and in the regulatory networks that shape brain development and function. Altered expression of NXPE4 has been reported in association studies across disease-relevant cohorts, motivating mechanistic studies to define how perturbation impacts cellular phenotypes.
FAM55D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NXPE4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NXPE4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NXPE4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM55D protein expression.
This CRISPR knockout system enables efficient generation of NXPE4-deficient cell models for investigation of FAM55D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NXPE4 exon(s) critical for FAM55D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NXPE4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM55D CRISPR/Cas9 KO Plasmid (h) and FAM55D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NXPE4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM55D HDR Plasmid (h) and FAM55D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NXPE4 homology arms to support homology-directed repair at defined NXPE4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.