FAM208A CRISPR/Cas9 KO Plasmid (h): sc-405884
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM208A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM208A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM208A CRISPR/Cas9 KO Plasmid (h) | sc-405884 | 20 µg | $397.00 |
Overview
FAM208A encodes a nuclear protein that participates in chromatin-associated processes influencing transcriptional regulation and genome maintenance. It has been linked to DNA damage response pathways and epigenetic control through interactions with multiprotein nuclear complexes, supporting proper cell-cycle progression and stress adaptation. Altered regulation of chromatin organization and repair fidelity involving FAM208A may contribute to genomic instability phenotypes observed in cancer and other proliferative disorders. Its predominantly nuclear localization makes it a useful target for dissecting mechanisms that couple chromatin state to gene expression and DNA repair outcomes in human cells.
FAM208A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM208A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM208A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM208A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM208A protein expression.
This CRISPR knockout system enables efficient generation of FAM208A-deficient cell models for investigation of FAM208A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM208A exon(s) critical for FAM208A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM208A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM208A CRISPR/Cas9 KO Plasmid (h) and FAM208A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM208A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM208A HDR Plasmid (h) and FAM208A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM208A homology arms to support homology-directed repair at defined FAM208A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.