Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FAM123A CRISPR/Cas9 KO Plasmid (h): sc-404231
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM123A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM123A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAM123A Antibody (G-1): sc-374654
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM123A CRISPR/Cas9 KO Plasmid (h) | sc-404231 | 20 µg | $397.00 |
Overview
AMER2 (FAM123A) is a cytoplasmic protein implicated in the regulation of Wnt/β-catenin signaling and cell polarity-associated processes, linking membrane-proximal cues to downstream transcriptional programs that influence proliferation and differentiation. By modulating components of the β-catenin regulatory network, FAM123A can affect adhesion and cytoskeletal organization, with potential impact on epithelial tissue architecture. Altered Wnt pathway activity is broadly relevant to developmental biology and oncogenic transformation, making AMER2 a useful entry point for dissecting signaling-state dependencies. Research on AMER2/FAM123A supports mechanistic studies of pathway crosstalk, context-specific transcriptional outputs, and genotype–phenotype relationships in human cell models.
FAM123A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AMER2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AMER2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AMER2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM123A protein expression.
This CRISPR knockout system enables efficient generation of AMER2-deficient cell models for investigation of FAM123A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AMER2 exon(s) critical for FAM123A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AMER2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM123A CRISPR/Cas9 KO Plasmid (h) and FAM123A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AMER2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM123A HDR Plasmid (h) and FAM123A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AMER2 homology arms to support homology-directed repair at defined AMER2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.