EYA1 Double Nickase Plasmid (h): sc-404056-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EYA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EYA1 Double Nickase Plasmid (h) and EYA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EYA1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA1 Double Nickase Plasmid (h) | sc-404056-NIC | 20 µg | $410.00 | |||
EYA1 Double Nickase Plasmid (h2) | sc-404056-NIC-2 | 20 µg | $410.00 |
EYA1 encodes Eyes Absent Homolog 1, a transcriptional coactivator and dual-specificity phosphatase that modulates developmental gene programs through interactions with SIX and DACH family proteins. EYA1 contributes to organogenesis-associated signaling networks, integrating phosphorylation-dependent control of transcription and cell fate decisions in pathways governing epithelial differentiation and progenitor maintenance. Dysregulated EYA1 function is linked to congenital disorders affecting craniofacial, auditory, and renal development, and altered expression has been investigated in contexts of tumor progression and cellular plasticity. These features make EYA1 a useful target for studying transcriptional circuitry, developmental biology, and phosphatase-regulated gene expression.
EYA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EYA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EYA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EYA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EYA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.