ERdj3 CRISPR/Cas9 KO Plasmid (h): sc-404935
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ERdj3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ERdj3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ERdj3 Antibody (C-7): sc-271240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERdj3 CRISPR/Cas9 KO Plasmid (h) | sc-404935 | 20 µg | $397.00 |
Overview
DNAJB11 encodes ERdj3, an ER luminal Hsp40 co-chaperone that partners with BiP/HSPA5 to promote folding of newly synthesized secretory and membrane proteins and limit aggregation. ERdj3 participates in endoplasmic reticulum proteostasis by stimulating BiP ATPase activity, coordinating quality control decisions linked to ER-associated degradation (ERAD), and modulating unfolded protein response (UPR) signaling during ER stress. Through these functions, DNAJB11 influences secretory pathway efficiency and cellular adaptation to misfolded protein load, processes implicated in disorders characterized by proteostasis imbalance, including kidney and hepatic pathophysiology as well as cancer-associated stress responses. Its role in chaperone networks makes it relevant for studying how ER stress integrates with apoptosis, inflammation, and metabolic remodeling.
ERdj3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJB11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJB11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJB11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ERdj3 protein expression.
This CRISPR knockout system enables efficient generation of DNAJB11-deficient cell models for investigation of ERdj3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJB11 exon(s) critical for ERdj3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJB11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ERdj3 CRISPR/Cas9 KO Plasmid (h) and ERdj3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJB11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ERdj3 HDR Plasmid (h) and ERdj3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJB11 homology arms to support homology-directed repair at defined DNAJB11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.