EpoR CRISPR/Cas9 KO Plasmid (m): sc-420210
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EpoR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EpoR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EpoR CRISPR/Cas9 KO Plasmid (m) | sc-420210 | 20 µg | $397.00 |
Overview
Epor encodes the erythropoietin receptor (EpoR), a type I cytokine receptor essential for erythroid progenitor survival, proliferation, and differentiation in mouse hematopoiesis. Ligand-induced receptor dimerization activates JAK2 and downstream STAT5 signaling, with additional coupling to PI3K–AKT and MAPK/ERK pathways that regulate anti-apoptotic and cell-cycle programs. EpoR activity shapes responses to hypoxia-driven erythropoietin signaling and integrates with broader cytokine networks in bone marrow and spleen. Dysregulated EPOR pathway signaling is widely used as a model for studying erythropoiesis defects, cytokine receptor signaling perturbations, and stress erythropoiesis mechanisms relevant to anemia and erythroid hyperproliferation phenotypes.
EpoR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Epor gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Epor together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Epor open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EpoR protein expression.
This CRISPR knockout system enables efficient generation of Epor-deficient cell models for investigation of EpoR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Epor exon(s) critical for EpoR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Epor genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EpoR CRISPR/Cas9 KO Plasmid (m) and EpoR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Epor locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EpoR HDR Plasmid (m) and EpoR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Epor homology arms to support homology-directed repair at defined Epor target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.