EphB6 Double Nickase Plasmid (h): sc-403979-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EphB6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EphB6 Double Nickase Plasmid (h) and EphB6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EPHB6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EphB6 Antibody (D-7): sc-398795
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EphB6 Double Nickase Plasmid (h) | sc-403979-NIC | 20 µg | $410.00 | |||
EphB6 Double Nickase Plasmid (h2) | sc-403979-NIC-2 | 20 µg | $410.00 |
EPHB6 encodes EphB6, a kinase-impaired member of the Eph receptor tyrosine kinase family that regulates cell–cell communication through ephrin-B ligand interactions. EphB6 participates in contact-dependent signaling that modulates cytoskeletal remodeling, adhesion, and directional migration, influencing processes such as epithelial organization and immune cell behavior. Through crosstalk with pathways including Rho GTPase signaling and MAPK/ERK and PI3K/AKT networks, EphB6 can shape downstream transcriptional programs and cell fate decisions. Altered EPHB6 expression has been associated with tumor progression and metastatic phenotypes in multiple cancer contexts, supporting its use as a target for mechanistic studies of invasion and microenvironmental signaling.
EphB6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EPHB6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EPHB6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EPHB6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EPHB6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.